•  High sensitivity, high specificity and accurate data
•  Passive reference dyes are suitable for different qPCR instruments

CITATIONS

Duan W, et al. 2013. Neuroprotection by Monocarbonyl Dimethoxycurcumin C: Ameliorating the Toxicity of Mutant TDP-43 via HO-1. Mol Neurobiol. IF=5.471. PMID: 23934646. 

Kong X, et al. 2013. Genome-Wide Identification and Analysis of Expression Profiles of Maize Mitogen-Activated Protein Kinase Kinase Kinase. 8(2):e57714. PLos One. IF=3.73. PMID: 23460898.

Li X, et al. 2013. Toxic effects of chlorpyrifos on lysozyme activities, the contents of complement C3 and IgM, and IgM and complement C3 expressions in common carp (Cyprinus carpio L.). 93(2):428-33. Chemosphere. IF=3.137. PMID: 23769463.

Li X, et al. 2013. Expression alterations of cytochromes P4501A1, 2E1, and 3A, and their receptors AhR and PXR caused by 1-octyl-3-methylimidazolium chloride in mouse mammary carcinoma cells. pii: S0045-6535(13)01249-6. Chemosphere. IF=3.137. PMID: 24094773.

Yang L. et al. 2013. Icariin from Epimedium brevicornum Maxim promotes the biosynthesis of estrogen by aromatase (CYP19). 145(3):715-21. J Ethnopharmacol.  IF= 3.014.  PMID: 23261485.

Li Q, et al. 2013. Effects of Simulated Microgravity on Primary Human NK Cells. 13(8):703-14. Astrobiology. IF=2.803. PMID: 23919749.

Jiang S, et al. 2013. A maize calcium-dependent protein kinase gene, ZmCPK4, positively regulated abscisic acid signaling and enhanced drought stress tolerance in transgenic Arabidopsis. 71C:112-120. Plant Physiol Biochem. IF=2.775. PMID: 23911729.

Lu D. et al. 2012. Egonol gentiobioside and egonol gentiotrioside from Styrax perkinsiae promote the biosynthesis of estrogen by aromatase. 691(1-3):275-82. Eur J Pharmacol.  IF= 2.516.  PMID: 22796452.

GC Enhancer can be used to increase sensitivity and specificity for GC/ATrich template or complex template. The tube contains 500 ul of GC enhancer.

Applications

• Complex templates

• GC/AT-rich templates

TransScript® First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The kit is ready for 50 reactions. The cDNA is efficiently synthesized by TransScript® RT/RI Enzyme Mix .

 Advantages

•  Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.

• Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.

• cDNA up to 12 kb

CITATIONS

Jia Q, et al. 2013. Transcriptome Analysis of the Zebrafish Model of Diamond-Blackfan Anemia from RPS19 Deficiency via p53-Dependent and-Independent Pathways. 8(8):e71782. PLoS One. IF=3.73. PMID: 23990987.

Zeng T, et al. 2013. Comparative Proteomic Analysis of the Hepatic Response to Heat Stress in Muscovy and Pekin Ducks: Insight into Thermal Tolerance Related to Energy Metabolism. 8(10):e76917.PLoS One. IF=3.73. PMID: 24116183.

Ni Y, et al. 2013. Responses of physiological indexes and leaf epicuticular waxes of Brassica napus to Sclerotinia sclerotiorum infection. Plant Pathol. IF=2.729.

Mao J, Zeng F. 2013. Plant-mediated RNAi of a gap gene-enhanced tobacco tolerance against the Myzus persicae. Transgenin Res.IF=2.609. PMID: 23949691.

Li X P, et al. 2013. Linolelaidic Acid Induces a Stronger Proliferative Effect on Human Umbilical Vein Smooth Muscle Cells Compared to Elaidic Acid. 48(4):395-403. Lipids.IF=2.557. PMID: 23341188.

Zhao W, et al. 2013. Molecular Cloning and Functional Analysis of the Duck TLR4 Gene. 14(9):18615-28. Int Mol Sci.IF=2.464. PMID: 24025421.

Tan C M, et al. 2013. OsPOP5, A Prolyl Oligopeptidase Family Gene from Rice Confers Abiotic Stress Tolerance in Escherichia coli. 14(10): 20204-20219.Int Mol Sci.IF=2.464.

Chi L, et al. 2013. Pepsinogen A and C genes in turbot (Scophthalmus maximus): Characterization and expression in early development. 165(1):58-65. Comp Biochem Physiol B Biochem Mol Biol.IF=2.069. PMID: 23499940.

Xu Y Y, et al. 2013. Cloning and expression analysis of P-glycoprotein gene in Crassostrea ariakensis. Aquaculture. IF=2.009.

Wang X. 2010. Characterization of genes expressed in response to cadmium exposure in the earthworm Eisenia fetida using DDRT-PCR. 73(6):1214-20. Ecotoxicol Environ Saf. IF= 2.294.  PMID: 20674023.

Zheng Z. 2012. Relative catalytic efficiency of ldhL- and ldhD-encoded products is crucial for optical purity of lactic acid produced by lactobacillus strains. 78(9):3480-3. Appl Environ Microbiol. IF= 3.829.  PMID: 22344644.

Zhang X. 2012. Role of plnB gene in the regulation of bacteriocin production in Lactobacillus paraplantarum L-XM1. Microbiol Res. IF= 2.308.  PMID: 23276644.

Chunhua M. 2012. The expression of LytM is down-regulated by RNAIII in Staphylococcus aureus. 52(6):636-41. J Basic Microbiol. IF= 1.266.  PMID: 22581788.

Wang L. 2011. Role of ghrelin on testosterone secretion and the mRNA expression of androgen receptors in adult rat testis. 57(3):119-23. Syst Biol Reprod Med. IF= 1.524.  PMID: 21204602.

Hu J. 2011. MiR-145 regulates epithelial to mesenchymal transition of breast cancer cells by targeting Oct4. 7(9):e45965. PLoS One. IF= 4.092.  PMID: 23049906.

Zhang FL. 2012. Hypoxia-inducible factor 1-mediated human GATA1 induction promotes erythroid differentiation under hypoxic conditions. 16(8):1889-99. J Cell Mol Med. IF= 4.125.  PMID: 22050843.

Xu X. 2012. Silencing of a novel tumor metastasis suppressor gene LASS2/TMSG1 promotes invasion of prostate cancer cell in vitro through increase of vacuolar ATPase activity. 113(7):2356-63. J Cell Biochem. IF= 2.868.  PMID: 22573553.

Zhang FL. 2011. Hypoxic induction of human erythroid-specific δ-aminolevulinate synthase mediated by hypoxia-inducible factor 1. 50(7):1194-202. Biochemistry. IF= 3.422.  PMID: 21207956.

Liu S. 2011. A multiplex RT-PCR for rapid and simultaneous detection of porcine teschovirus, classical swine fever virus, and porcine reproductive and respiratory syndrome virus in clinical specimens. 172(1-2):88-92. J Virol Methods. IF= 2.011.  PMID: 21192983.

Mao J. 2012. Feeding-based RNA interference of a gap gene is lethal to the pea aphid, Acyrthosiphon pisum. 7(11):e48718. PLoS One. IF= 4.092.  PMID: 23144942.

Sun N. 2012. Localization, expression change in PRRSV infection and association analysis of the porcine TAP1 gene. 8(1):49-58. Int J Biol Sci. IF= 2.699.  PMID: 22211104.

Tan X. 2012. Clock-controlled mir-142-3p can target its activator, Bmal1. 13:27. BMC Mol Biol. IF= 2.857.  PMID: 22958478.

Gray WD. 2013. Dendrimeric bowties featuring hemispheric-selective decoration of ligands for microRNA-based therapy. 14(1):101-9. Biomacromolecules. IF= 5.479.  PMID: 23145944.

Zhang B. 2011. NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis. 68(5):830-43. Plant J.  IF= 6.16.  PMID: 21801253.

Fang F. 2012. Role of ghrelin on estrogen and progesterone secretion in the adult rat ovary during estrous cycle. 58(2):116-9. Syst Biol Reprod Med.  IF= 1.524.  PMID: 22142178.

Zu M. 2012. In vitro anti-influenza virus and anti-inflammatory activities of theaflavin derivatives. 94(3):217-24. Antiviral Res.  IF= 4.301.  PMID: 22521753.

Jiang L. 2012. Identification and expression of C2H2 transcription factor genes in Carica papaya under abiotic and biotic stresses. 39(6):7105-15. Mol Biol Rep.  IF= 2.929.  PMID: 22484790.

Longxi P. 2011. Expression of p53 in the effects of artesunate on induction of apoptosis and inhibition of proliferation in rat primary hepatic stellate cells. 6(10):e26500. PLoS One. IF= 4.092.  PMID: 22053192.

Pan B. 2012. High-density lipoprotein of patients with type 2 diabetes mellitus elevates the capability of promoting migration and invasion of breast cancer cells. 131(1):70-82. Int J Cancer. IF= 5.444.  PMID: 21805479.

Yu X. 2012. Assessment of the treatment effect of baicalein on a model of Parkinsonian tremor and elucidation of the mechanism. 91(1-2):5-13. Life Sci. IF= 2.527.  PMID: 22634324.

Jiang LY. 2013. The effects of an RGD-PAMAM dendrimer conjugate in 3D spheroid culture on cell proliferation, expression and aggregation.34(11):2665-73.Biomaterials. IF= 7.404.  PMID: 23340194.

EasyPure® RNA Kit provides a fast and easy column based method to isolate total RNA from animal cells, animal tissues, bacteria, and yeast in 30-40 minutes. Cells and tissues are enzymatically lysed. DNA is digested with DNase I. RNA is bound to silica membrane. After washing, high quality RNA is eluted from the column. Isolated RNA is free of DNA and protein contamination, and is suitable for RT-PCR, qRT-PCR and Northern blot.

CITATIONS

Wang B, et al. 2013. Evolution and Expression Plasticity of Opsin Genes in a Fig Pollinator, Ceratosolen solmsi. 8(1):e53907. PLoS One. IF=3.73. PMID: 23342036.

Li H, et al.2013. Nspc1 regulates the key pluripotent Oct4-Nanog-Sox2 axis in P19 embryonal carcinoma cells via directly activating Oct4. pii: S0006-291X(13)01600-8. Biochem Biophys Res Commun. IF=2.406. PMID: 24113379.

Zhao W, et al. 2013. Heat-induced oxidative injury contributes to inhibition of Botrytis cinerea spore germination and growth. World J Microbiol Biotechnol. IF=1.262. PMID: 24101365.

PCR Stimulant can be used to increase sensitivity and specificity for GC/ AT-rich template or complex template. It is especially suitable for Pfu enzymes. The stock concentration is 5×, and the working concentration can be varied between 0.5× and 2.5×. The tube has 200 ul of PCR stimulant.

The 100bp DNA Ladder is composed of eleven linear double-stranded DNA bands. This ready-to-use product is premixed with 1×DNA loading buffer. Based on experiment requirement, 5 μl marker can be directly loaded for gel electrophoresis. It provides simple operation and produces clear image. For easy reference on agarose gels, the 500 bp band (100 ng/5 μl) is brighter than the other bands (50 ng/5 μl).

CITATIONS

Hu X, et al. 2013. Molecular Cloning and Functional Characterization of the Dual Oxidase (BmDuox) Gene from the Silkworm Bombyx mori. 8(8):e70118. PLoS One. IF=3.73. PMID: 23936382.

Wu SF. et al. 2013. Molecular cloning and pharmacological characterisation of a tyramine receptor from the rice stem borer, Chilo suppressalis (Walker). 69(1):126-34. Pest Manag Sci. IF= 2.594.  PMID: 23129510.

1 Kb DNA Ladder is composed of eight linear double-stranded DNA bands. This ready-to-use product is premixed with 1×DNA loading buffer. Based on experiment requirement, 5 μl marker can be directly loaded for gel electrophoresis. It provides simple operation and produces clear image. For easy reference on agarose gels, the 4000 bp band (100 ng/5 μl) is brighter than the other bands (50 ng/5 μl).

Trans2K® DNA Marker is composed of six linear double-stranded DNA bands. This ready-to-use product is premixed with 1x DNA loading buffer. Based on experiment requirement, 5 μl marker can be directly loaded for gel electrophoresis. It provides simple operation and produces clear image. For easy reference on agarose gels, the 750 bp band (100 ng/5 μl) is two times brighter than the other bands (50 ng/5 μl) in the ladder.

CITATIONS

Hu X, et al. 2013. Molecular Cloning and Functional Characterization of the Dual Oxidase (BmDuox) Gene from the Silkworm Bombyx mori. 8(8):e70118. PLoS One. IF=3.73. PMID: 23936382.

Wu SF. et al. 2013. Molecular cloning and pharmacological characterisation of a tyramine receptor from the rice stem borer, Chilo suppressalis (Walker). 69(1):126-34. Pest Manag Sci. IF= 2.594.  PMID: 23129510.

Trans5K DNA Marker is composed of eight linear double-stranded DNA bands. This ready-to-use product is premixed with 1 × DNA loading buffer. Based on experiment requirement, 5 μl marker can be directly loaded for gel electrophoresis. It provides simple operation and produces clear image. For easy reference on agarose gels, the 1500 bp band (125 ng/5 μl) is brighter than the other bands (50 ng/5 μl).

CITATIONS

Hu X, et al. 2013. Molecular Cloning and Functional Characterization of the Dual Oxidase (BmDuox) Gene from the Silkworm Bombyx mori. 8(8):e70118. PLoS One. IF=3.73. PMID: 23936382.

Wu SF. et al. 2013. Molecular cloning and pharmacological characterisation of a tyramine receptor from the rice stem borer, Chilo suppressalis (Walker). 69(1):126-34. Pest Manag Sci. IF= 2.594.  PMID: 23129510.

Trans15K DNA Marker is composed of eight linear double-stranded DNA bands. This ready-to-use product is premixed with 1x DNA loading buffer. Based on experiment requirement, 5 μl marker can be directly loaded for gel electrophoresis. It provides simple operation and produces clear image. For easy reference on agarose gels, the 5000 bp band (100 ng/5 μl) is brighter than the other bands (50 ng/5 μl).

CITATIONS

Wang Y. et al. 2011. Detection of the staphylococcal multiresistance gene cfr in Proteus vulgaris of food animal origin.66(11):2521-6. IF= 5.068.  PMID: 21795256.

Liu Y. et al. 2012. First report of the multidrug resistance gene cfr in Enterococcus faecalis of animal origin.56(3):1650-4. IF= 4.841.  PMID: 22203597.

RNAhold is an aqueous, nontoxic tissue preservation solution. It can inactivate RNase and keep RNA intact by permeating cells and tissues. Cells and tissues can be stored at this solution for one week at room temperature without RNA degradation. It is suitable for fresh and nonfrozen tissue from E.coli, cells and a variety of animals.

Ampicillin is commonly used as a selective antibiotic for resistant bacteria.

Kanamycin is commonly used as a selective antibiotic for resistant bacteria.

Chloramphenicol is commonly used as a selective antibiotic for resistant bacteria.

6×DNA Loading Buffer is used  as loading buffer to prepare DNA markers and samples for on agarose gel electrophoresis. Appropriate volume of 6×DNA Loading Buffer is added into DNA samples to make its working concentration at 1×, and then the DNA samples can be loaded on the gel for electrophoresis.