~~Deoxyribonuclease I (DNase I) digests single- and double-stranded DNA to oligodeoxyribonucleotides containing a 5’ phosphate. Ribonuclease has been reduced to non-detectable levels. Its activity depends on Mg2+ or Mn2+ ion. DNase I with Mg2+ cuts randomly double strand DNA at any sites, DNase I with Mn2+ cuts double strand DNA at the same site to form sticky-end with 1-2 nucleotide or blunt-end.

